Vector design, construction, and production
The large amount of viral vector preparation is made possible by transfection of several plasmids into cells, including one transfer plasmid and two or more packaging plasmids. The TFBS team provides services of vector design, plasmid construction, and plasmid production to support the first step of product development. TFBS transfection platform of lentivirus and AAV has been validated by using the in-house viral vector plasmid system.
To fulfill the regulatory requirement, all cell banks used to produce plasmids need to be established in controlled areas, which should also be characterized according to regulatory guideline. TFBS is able to perform all these activities in house or with our collaboration partners.